Part:BBa_K777133

Similar to pSB1C3, with RFP reporter

This part is similar to J61002 but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.

Usage and Biology

This construct can be useful in different ways, just like the original part J61002. However, it is based on pSB1C3 and carries a chloramphenicol resistance.

1. You may use this plasmid to insert a promoter of your choice between the XbaI/EcoRI and SpeI sites. This will provide you with a downstream mRFP as a reporter. Look up the specifications for restriction sites here.
2. Furthermore, you can clone a gene of interest downstream of the constitutive Anderson promoters to express it at a desired rate. For this, the SpeI and PstI sites can be used. Look up the specifications for restriction sites here.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2917
  Illegal XbaI site found at 2932
  Illegal SpeI site found at 1
  Illegal PstI site found at 884
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2917
  Illegal SpeI site found at 1
  Illegal PstI site found at 884
  Illegal NotI site found at 877
  Illegal NotI site found at 2923
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2917
  Illegal XhoI site found at 1901
  Illegal XhoI site found at 2793
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a suffix.
  Illegal SpeI site found at 1
  Illegal PstI site found at 884
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2917
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2932
  Illegal SpeI site found at 1
  Illegal PstI site found at 884
  Illegal AgeI site found at 580
  Illegal AgeI site found at 692
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  

Fig.1: Plasmid map of K777133